What is the effect of Nickel (II) Chloride concentration on …
The student provides a focused and detailed description of the main topic, including a clear aim to investigate the effects of varying concentrations of nickel (II) chloride on the germination rate of Triticum aestivum. The background information provided is relevant and well-organized, covering essential concepts such as bioaccumulation and ...
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Effect of mechanical stretching on DNA conductance
Here we study the effect of mechanical stretching-induced structural changes on charge transport in single DNA molecules. The charge transport follows the hopping mechanism for DNA molecules with lengths varying from 6 to 26 base pairs, but the conductance is highly sensitive to mechanical stretching, showing an abrupt decrease at …
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DNA intermediates at the Escherichia coli replication fork: effect …
We have directly tested the hypothesis that elevated levels of dUTP cause the formation of small DNA fragments at the replication fork of Escherichia coli. Addition of increasing levels of dUTP to lysates on cellophane discs results in an increasing number of strand scissions in the newly replicated DNA. Lysates of strains defective in dUTPase ...
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EFFECT OF CONSCIOUS INTENTION ON HUMAN DNA
The effects observed here on DNA synthesis were shown to be dependent on the intention of the healer with some intentions producing larger effects and others producing effects in . 3 the opposite direction. It was also demonstrated that imagery as well as intention as a …
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Effect of Aphidicolin, a Reversible Inhibitor of Eukaryotic Nuclear DNA
There is no previous information on the use of aphidicolin in porcine embryos, so the reversible inhibition of DNA replication and the effect on embryo development of different concentrations of this drug was first evaluated. The effect of incubation with aphidicolin was tested with CRISPR/Cas9 at different concentrations and …
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Synthesis, Spectroscopy and Biological studies of Nickel
Request PDF | Synthesis, Spectroscopy and Biological studies of Nickel(II) complexes with tetradentate Schiff Bases having N 2 O 2 donor group | Complexes of nickel(II) of N,N'-disalicylidene-3,4 ...
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Effect of static magnetic field on DNA synthesis: The
Here we report on the chirality-driven effect of static magnetic fields (SMFs) on DNA synthesis. This newly discovered effect reveals how the interplay between two fundamental features of symmetry in living and inanimate nature-DNA chirality and the inherent features of MFs to distinguish the left and right-manifests itself in different DNA ...
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The Effects of Dithiothreitol on DNA
DTT was furthermore shown to facilitate the immobilization of fluorescent DNA on an NHS-ester functionalized glass surface. Such reactions may in particular impact the readout from single molecule detection studies and other ultrasensitive assays. This was highlighted by the finding that DTT markedly decreased the signal to noise ratio in a …
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Oxidative modification enhances the immunostimulatory effects …
One of these endogenous danger signals is the extracellular mitochondrial DNA (mtDNA) containing evolutionary conserved unmethylated CpG repeats. ... (HLA-DQ) on the cell surface, as well as increased TNF-α and IL-8 production from the cells. These effects were more apparent when pDCs were exposed to oxidatively modified mtDNA. Neither native ...
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Effects of chlorine dioxide on the germination, oxidative
The effects of chlorine dioxide, ClO2, on the germination, oxidative metabolism and growth of barley seedlings were investigated. Barley seeds were separately treated with 0, 500, 1000 and 2000 mg ...
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DNA methylation mediates the effects of PM2.5 and O3 on …
Mediation analysis was subsequently performed to evaluate the potential mediating effect of DNA methylation in the association between air pollution and ceramide metabolism. PM 2.5 and O 3 were associated with elevated IR. Specifically, each 10 μg/m 3 increase in PM 2.5 and O 3 at lag0–12 h significantly increased triglyceride‑glucose ...
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Position-dependent effects of locked nucleic acid (LNA) on DNA
Locked Nucleic Acid (LNA) can enhance oligonucleotide affinity and specificity. Though LNA has been used in many applications, formal design rules are still being defined. To further this effort we have investigated the effect of LNA on the performance of sequencing and PCR primers in AT-rich regions, where short primers yield poor sequencing ...
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DNA | Definition, Discovery, Function, Bases, Facts, & Structure
The configuration of the DNA molecule is highly stable, allowing it to act as a template for the replication of new DNA molecules, as well as for the production (transcription) of the related RNA (ribonucleic acid) molecule.A segment of DNA that codes for the cell's synthesis of a specific protein is called a gene.. DNA replicates by …
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Effects of nickel on DNA methyltransferase activity and genomic DNA
These results suggest that nickel exposure can elevate total genomic DNA methylation levels even when DNA methyltransferase activity is depressed. These findings may explain the hypermethylation of senescence and tumor suppressor genes found during nickel carcinogenesis and support the model of a direct effect of Ni2+ on chromatin …
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A systematic review and meta-analysis to determine the effect
Sperm DNA damage is prevalent among infertile men and is known to influence natural reproduction. However, the impact of sperm DNA damage on assisted reproduction outcomes remains controversial. Here, we conducted a meta-analysis of studies on sperm DNA damage (assessed by SCSA, TUNEL, SCD, or Comet …
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Structural, Electrochemical, DNA Binding and Cleavage …
DNA can cause DNA damage in cancer cells, blocking the division of cancer cells and resulting in cell death.4,5 The cleavage of nucleic acids may be considered as an enzymatic
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Effects of pH, conductivity, host cell protein, and DNA size
The DNA binding capacity values measured in this study were lower compared to published spiking studies on AEX chromatography membranes using commercial herring sperm DNA (hs‐DNA).11, 12 The differences could be attributed to several factors including operational conditions and the different types of DNA used for …
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Exploring the binding and cleavage activities of nickelII …
The present study documents the first investigation into the relationship between the binding and cleavage activity of nickel complexes towards DNA and proteins. Here, three different NiII complexes of the general type [Ni(nal)2diimine] (1a and 1b) and [Ni(8-Hq)2(bpy)] 1c (nal = nalidixic acid, diimine = 2,2
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Nickel (II)-induced apoptosis and G2/M …
These findings suggest that nickel (II) can modulate cellular response through some common effectors involving in both apoptotic and cell cycle regulatory pathways. …
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Effect of Temperature on the Intrinsic Flexibility of DNA …
Double-stranded DNA (dsDNA) is a semiflexible polymer. 1 On short length scales (on the order of its persistence length, ∼150 bp or ∼50 nm), stiffness dominates its conformation and bending is energetically unfavorable. However, on a larger scale, dsDNA acts as a flexible polymer and forms a random coil. Although the exact cause of DNA …
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The effects of tissue fixation alternatives on DNA content: …
Abstract. The aim of this study is to investigate the effects of different fixatives on DNA, and to evaluate the fixation options for molecular studies including polymerase chain reaction (PCR) and fluorescence in-situ hybridization (FISH). Three normal-looking colonic segments from surgical resections were used for tissue sampling.
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Effects of Ultraviolet Radiation on DNA | SpringerLink
Abstract. UV radiation is selectively absorbed by DNA, mainly in the UV-B and the UV-C regions. Excitation of DNA leads to typical photoproducts, the most important being pyrimidine dimers and the so-called 6-4-photoproduct. The yield of strand breaks is very low directly after exposure, but they may be formed indirectly upon further incubation.
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Effect of nickel (II) on DNA-protein binding, …
Treatment of cells with nickel chloride (NiCl2) decreased the amount of DNA in the magnesium-insoluble fraction but increased the amount of DNA in the fast-sedimenting …
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Effect of nickel chloride on seed germination and
The effect of nickel chloride concentrations in the range of 1–1000 mg/L on seed germination and the development of organs and pigment system in seedlings of the helophyte common water-plantain Alisma plantago-aquatica L. and hygrohelophyte greater water-parsnip Sium latifolium L. has been studied. It is shown that nickel chloride has …
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Effects of DNA supercoiling on chromatin architecture
We propose a model in which changes in unconstrained DNA supercoiling influences higher levels of chromatin organisation through the additive effects of DNA supercoiling on both DNA-protein and DNA-nucleosome interactions. This model links small-scale changes in DNA and chromatin to the higher-order fibre and large-scale chromatin structures ...
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Dr Ambedkar Center for Biomedical Research
Exploring the binding and cleavage activities of nickelII complexes towards DNA and proteins, M Kumar, N Lal, PM Luthra and DT Masram, New Journal of Chemistry (RSC), 2021 https: ... Neuroprotective effect of IDPU (1-(7-imino-3-propyl-2,3-dihydrothiazolo [4,5-d]pyrimidin-6(7H)-yl)urea) in 6-OHDA induced rodent model of hemiparkinson's disease, ...
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Effect of nickel(II) on DNA-protein interactions
14 Citations. Explore all metrics. Abstract. Alterations in DNA-protein interactions (DPI) may play an important role in carcinogenesis. Although the mechanism of nickel …
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The Effects of RNA.DNA-DNA Triple Helices on Nucleosome …
Biochemical experiments have shown that ncRNA binding to nucleosomal DNA is elevated at DNA entry and exit sites and is dependent on the presence of the H3 N-terminal tails. However, the structural and dynamical bases for these mechanisms remains unknown. Here, we have examined the mechanisms and effects of RDD formation in the context of …
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Exploring the Molecular Mechanisms of Nickel …
Experimental studies have shown that nickel disrupts DNA strands, DNA crosslinks, and DNA repair. Oxidative stress has been found to play a key role in nickel-induced carcinogenicity / 47, 48 /. The production of reactive oxygen species (ROS) is …
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Effect of structure and composition of nickel(II) …
Effect of structure and composition of nickel (II) complexes with salicylidene Schiff base ligands on their DNA/protein interaction and cytotoxicity. J Inorg Biochem. 2014 …
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